This work was done at the University of North Carolina, Chapel Hill using a quartz chamber AutoGlow:
The PCL substrates were modified by oxygen plasma treatment in an AutoGlow oxygen plasma system by Glow Research. Oxygen pressure was maintained at 1.3 mbar while the PCL substrates were subjected to 30 W of power intensity for 1 min. Prior to cell seeding, films were sterilized with UV light for 10 min per side. The PCL films were conditioned first by incubating in PBS buffer (1X) for 2 h. Subsequently, the substrates were immersed in 1 mL of 0.005 mg/mL fibronectin solution for 1 h. Surface hydrophobicity was evaluated by static water contact angle measurements. Contact angle was acquired using a KSV Instruments Cam 200 Optical goniometer using the sessile drop method.
Dynamic Topographical Control of Mesenchymal Stem Cells by Culture of Responsive Poly (e-caprolactone) Surfaces